human nectin 2  (Novoprotein)

Journal: Frontiers in Immunology

Article Title: Osteoclasts affect the anti-cancer activity of NK cells

doi: 10.3389/fimmu.2026.1730283

Figure Lengend Snippet: Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

Article Snippet: After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA).

Techniques: Expressing, Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Osteoclasts affect the anti-cancer activity of NK cells

doi: 10.3389/fimmu.2026.1730283

Figure Lengend Snippet: Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

Article Snippet: After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA).

Techniques: Co-Culture Assay, Expressing

Journal: Cell Discovery

Article Title: Stromal architecture and fibroblast subpopulations with opposing effects on outcomes in hepatocellular carcinoma

doi: 10.1038/s41421-024-00747-z

Figure Lengend Snippet: a UMAPs showing tissue origin of fibroblasts from human perturbed-state fibroblasts atlas (left) and their expression of CAF-FAP (middle) and CAF-C7 (right) signature. b Schematic diagram of integrating scRNA-seq data of human fibroblasts collected from different cancer types and locations. c Estimated CAF-FAP (upper) and CAF-C7 (lower) abundance in pan-cancer fibroblasts, showing the expression of subcluster signature among cancer types. d Pearson correlation between CAF-FAP and CD8_PDCD1 abundance and between FAP and PDCD1 gene expression of TCGA data across 32 solid tumor types. Cell abundance was quantified by mean expression of the top 50 DEGs. Pearson correlation. e Kaplan-Meier plot showing the overall survival across 6 cancer types from TCGA based on the mean expression of TIGIT and NECTIN2 . Log-rank test. f Characterization of the inflammatory hubs in published ST data of human breast, ovarian, and endometrial cancer, showing paired histology, spatial distribution, and correlation of CAF-FAP and CD8_PDCD1, pathway activity, marker gene expression, and ligand‒receptor pairs. Pearson correlation. g Schematic diagram of the two major stromal archetypes (FR + and FR ‒ ) in HCC, displaying broad differences between FR + and FR ‒ tumors regarding fibroblast heterogeneity, multicellular interactome, and ECM properties.

Article Snippet: NECTIN2 -overexpressing ( NECTIN2 -OE) and control LX-2 cells were cultured in confocal dishes and stained with DIO (MCE) 24 h post-transfection.

Techniques: Expressing, Gene Expression, Activity Assay, Marker